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HPV-DNA Test

General aspects

The Cervical Cancer is recognized as a first solid viral tumor, identified in women. In the global population the DNA of HPV (Human Papilloma Virus) is present in all carcinomas of the cervix and in the previous lesions (99.7% of cases). HPV is a common virus, transmitted sexually and usually it not give any disorder because like other virus (cold, flu) it is eliminated by the immune system. In the population, in some individuals the infection is persistent, so they not eliminate the virus that becomes cause of injury, responsible of a cervical cancer.

Genetic Counseling

The HPV virus are not all alike; until today it has been identified more than 100 different types, classified as "low risk” and at "high risk" of the cervical cancer occurrence. Each viral genotype is identified by the letters HPV followed by a number (eg. HPV 33, HPV 45, etc.). The genotypes HPV 16 and HPV 18 are responsible for over of 70% of cases of cervical cancer, so they are considered as a "high risk" oncogenic genotypes.

Genetic test

The HPV-DNA testing used in Prenatal Tecnobios Eurogenlab allows to highlight the presence of the DNA of 14 types of high risk HPV and define the genotype for HPV 16 and
HPV 18/45.
Table 1 lists the HPV genotypes.

High Risk Genotypes

16,18,31,33,35,39,45,51,52,56,58,59,66,68

Table 1: HPV High Risk Genotypes

An individual can be a carrier of two or more different HPV genotypes (coinfection).
Technically, the test use an amplification throughout a Real Time PCR of E6/E7 region of the viral genome.

Response times

The test results are available after 3-5 days from the sampling.

Analyzed material

DNA extracted from uterine cervix and oropharyngeal mucositis cells obtained with dry swab or with liquid phase cytology (thinprep, etc.). The test can be made also on the DNA of male individual, extract from the urethral or balano-preputial swab or seminal fluid.

Reservations

To performe the HPV-DNA test it’s necessary sampling revservation at Caravelli laboratory, Bologna. Phone: 051231531.

References

Munoz et al. - Epidemiologic Classification of Human Papilloma Types Associated with Cervical Cancer -N Engl J Med (2003)
Del Mistro et al. – Human papillomavirus typing of invasive cervical cancer in Italy. – Infect Agent Cancer (2006)
Wheeler et al - Human papillomavirus genotypes and the cumulative 2-year risk of cervical precancer. – J Infect Dis (2006)
Walboomers et al. – Human Papillomavirus is a necessary cause of invasive cervical cancer worldwide.- J Pathol (1999)
De Sanjose S, Quint WG, Alemany L, et al. - Human papillomavirus genotype attribution in invasive cervical cancer: a retrospective cross-sectional worldwide study. The Lancet (2010)
Li N, Franceschi S, Howell-Jones R, Snijders PJF, Clifford GM. - Human papillomavirus type distribution in 30,848 invasive cervical cancers worldwide: Variation by geographical region, histological type and year of publication. Int J Cancer (2011)